Sort Recovery Page

Version 5.0 Yield=exp(-(1-S)*r*T-r*t)
For the BDIS TurboSort® systems with a 0.1 drop clock cycle only
  Input Parameters   Output Parameters   
  Cell Sorter: Vantage SE Digital
Vantage/Plus 		  Deadtime Loss:  %   
  Mode: Normal-R1 Count    Throughput:  hr   
  Sort Envelope:  drops (purity mask)   Sort Left   
  Sample Flow Rate:  events/sec    Rate  /hr  /sec   
  Drop Frequency:  Hz    Yield  hr  min  %   
  Dead Time:   µS    Sort Right   
  Sort fractions: Left: %  Right:  %    Rate  / hr  /sec   
  Desired Yield: Left: Right:    Yield hr  min   %   
  Max Sample Loss:  % of sort left subset   Max: / secdrops/event   
  Action:    
 

 

These calculations apply to BDIS sorters where the instrument is set to trigger on light scatter. These calculations assume ideal conditions and that there are no other hidden dead times within the sorting electronics. In practice, there are internal constraints within some sort circuits that will cause additional aborts that may or may not be counted by the electronics. For high speed enrichment, everything changes based on signal/noise and other sample characteristics. Your mileage may vary... always check your instruments sorting efficiency...

1 When Normal-R is used, fractional drop sorting may actually give slightly better yields than predicted, since a 1.5 drop envelope may clock down to to 1.0 drop if no contaminating cell will be included within the single drop containing the cell of interest. The actual yield for a 1.4 drop sort envelope will usually be between that of a 1.4 drop and a 1.0 drop sort envelope using Normal-R.

Updated 20040430 - dc